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Description
ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.
Features
Contains all components for reverse transcription
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
Application
Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.
Storage
-20°C for 24 months
High yield
High sensitivity
Thermostable, up to 50°C, during first strand synthesis
Contents
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Storage Buffer
Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol
RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol
5X RT buffer (DTT)
250 mM Tris-HCl (pH 8.3), 50 mM DTT, 375 mM KCl 15 mM MgCl2 and 50 mM DTT
Storage
-20°C for 24 months
Manual
Manual_RP1300_ExcelRT™ Reverse Transcription Kit
SDS
SDS_RP1300
What is the difference between ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400)?
ExcelRT™ Reverse Transcription Kits contain all components to synthesize high quality first strand cDNA. The components in ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400) are the same.
The components in RP1300 are separated. This make RP1300 more flexible to fit different experimental requirements.
Selected components in RP1400 are pre-mixed. This make RP1400 more convenient to use.
ExcelRT™ Reverse Transcription Kit (RP1300) contains Random hexamers and Oligo(dT)20, how do I choose?
You can choose oligo d(T)20, Random hexamers, or both depending on the RNA template and the downstream applications.
Oligo (dT)20 anneals to poly(A) tails of eukaryotic mRNAs. It is suitable for cDNA libraries construction from eukaryotic mRNAs, full- length cDNA cloning, and 3′ rapid amplification of cDNA ends (3′ RACE). It is not suitable for cDNA synthesis from degraded RNA or RNAs that lack poly(A) tails, such as prokaryotic RNAs and microRNAs.
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[RP1400] ExcelRT™ Reverse Transcription Kit II
Contains all components for reverse transcription
High yield
Thermostable, up to 50°C Reduced RNase H ribonuclease activity
Suitable for real-time PCR
[TF1000] SMO-HiFi™ DNA Polymerase
5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
High reaction rate—10 seconds/kb
High fidelity— 70 times higher than Taq polymerase
Generates blunt end amplicon
Thermo-stable for more than 10 hrs at 95°C.
[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)
High sensitivity and signal intensity
Compatible with fast PCR program
With smart blue contrast dye as a visual aid for reaction setup
Low background
High stability
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix
5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
