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商品详细SMOBIO/[CC0202] Champion™ 109 High, 80 tubes/s)"}]]>

SMOBIO/[CC0202] Champion™ 109 High, 80 tubes/s)"}]]>

商品编号： CC0202

品牌： SMOBIO

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：克隆试剂盒

公司分类： Cloning_kit

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

Champion™ Competent Cells
pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C.

Storage and expiration

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

Genotypes and applications

Product Name

Genotype

Application

Champion™ 109 High

e14(McrA-)recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 thi-1 relA1 ∆(lac-proAB) (F’traD36 proAB lacIqZ∆M15

Appropriate for blue-white color and robotic screening. It is a fast growing strain forming visible colonies within 8~10 hours.

Champion™ 21

F’ ompT hsdSβ(rβ-mβ-) dcm gal λ (DE3)

Appropriate host for recombinant protein expression using T7-based expression vectors.

Champion™ DH5α High

recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 relA1 thi-1 ∆(lacZYA-argF)U 169 φ80 ∆(lacZ)M15 F’

Suitable for cloning with large plasmid and cDNA library construction, and also for blue-white colony selection.

Items and ordering information

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

>1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion™ 21

E. coli BL21 (DE3)

>1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion™ DH5α High

E. coli DH5α

>3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

Manual

Manual_Champion™ Competent Cell

SDS

SDS_Champion™ Competent Cell

Flyer

Champion™ Competent Cell

Protocol card

Will transformation efficiency be reduced when Champion™ competent cells are thawed, dispensed and refrozen repeatedly?

When Champion™ competent cells are thawed dispensed in aliquots and refrozen within 1 min, the transformation efficiency will be 10~20% reduced compared to the first time use.

What is the advantage of thawing competent cells with circulating water instead of still water?

Using circulating water can reduce the thawing time. Less thawing time shows higher efficiency than long thawing time.

Is there any difference of transformation efficiency between using plating beads, bending glass rod and plating loop?

The bending glass rod shows best efficiency and the plating loop show less efficiency than other method. For handle a lot samples at the same time the plating beads are recommended.

Is 1 second vortex critical for mixture the DNA?

One second vortex provides more reliable transformation efficiency (1.1 times compare with mixed by finger tapping)

Will heat shock affect the transformation efficiency?

Heat shock treatment will enhance the efficiency about 1~2X versus non-heat shock method.

Thawing methods

Shorter thawing time is more efficient than a longer thawing time. Slow thawing caused by power shortages and unstable freezers will result in decreased efficiency.

Size of plasmid

Plasmid size affect the efficiency greatly. The efficiency of transforming a supercoiled 2.7 kb is approximately 100 times higher than that of a 10 Kb plasmid (using time-saving transformation protocol). For large plasmids (> 6 kb), standard transformation protocol is recommended.

Heat shock treatment

Heat shock treatment will enhance the efficiency about 1~2 folds versus non-heat shock method.

Plating methods

Bent glass rods show the greatest efficiency, while plating loop shows less efficiency than plating beads. When handling a large quantity of samples at the same time, plating beads are recommended.

Concentration of antibiotic

Antibiotic concentration is critical to use of the time-saving transformation protocol.

Antibiotic

Concentration

Ampicillin (Ap)

20 μg/ml

Kanamycin (Km)

25 μg/ml

Tetracycline (Tc)

7.5 μg/ml

Chloramphenicol (Cm)

20 μg/ml

For plasmid size <6 Kb, the efficiency of kanamycin selection is usually 3~10 times less than the ampicillin selection. For plasmid size > 6 Kb, the efficiency of kanamycin selection is much lower than ampicillin. We suggest using the standard transformation protocol (with heat shock and recovery steps) to enhance the efficiency.

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

>1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion™ 21

E. coli BL21 (DE3)

>1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion™ DH5α High

E. coli DH5α

>3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)
[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.

Safe fluorescent dyes

[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

Blue-light illuminator

[VE0100] B-BOX™ Blue Light LED Epi-illuminator

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.

[CV1000] GetClone™ PCR Cloning Vector, 20 Rxn
[CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn

Colony PCR

Analyze colonies with PCR master mix to save preparation time.

[TP1100] ExcelTaq™ 5X PCR Master Mix, 200 Rxn
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix, 200 Rxn

品牌介绍

SMOBIO的B-BOX™是一种长波长蓝光LED落射照明器。它设计紧凑，结构坚固。B-BOX™落射照明器由于其非紫外线光源和低至12伏的低工作电压，并且为非致癌DNA /蛋白质染料提供了工作能力，因此为用户提供了前所未有的安全性。B-BOX™带有高质量的非闪烁LED灯，对DNA和蛋白质染料高度敏感。B-BOX™可以极大地减轻凝胶提取的日常琐事，即使在明亮的环境光下也可以轻松进行可视化和凝胶切割。多角度滤板（目录号VE0102）为凝胶切割，可视化和记录提供了最佳角度。B-BOX™还带有用于过滤和可视化的琥珀色滤镜（目录号VE0103）。Phox™Photobox（目录号 VE0104）为使用任何智能手机拍摄的图像提供了一个迷你暗室环境。最后，工作区域周围的内置屏障系统有助于清洁。特征提高克隆效率紧凑，轻巧和便携式（重量不足1千克）安全功能包括：470 nm长波长，对用户没有任何紫外线辐射危害与非致癌，非溴化乙锭的DNA染料兼容用户友好：样品易于观察（使用滤板或护目镜时） LED光源持续长达50,000小时出色的检测灵敏度：使用FluoroStain™DNA荧光染色染料时，DNA≤0.04 ng，使用FluoroStain™蛋白荧光染色染料时，蛋白≤3ng（与银染一样灵敏）可调和可移动的滤板可进行凝胶切割，可视化和记录内置屏障设计，易于清理在明亮的环境光下可见借助其自身的内置散热器，强调了最小的功率依赖，低发热

公司介绍

品牌分类

DNA梯子蛋白质标记物前盖尔斯 n. 照明者; n. 核酸; cDNA合成和RT-PCR 蛋白质污渍 DNA扩增; n. 无性（繁殖）系化，纯系化;

DNA染料

显示分类

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Will transformation efficiency be reduced when Champion™ competent cells are thawed, dispensed and refrozen repeatedly?

What is the advantage of thawing competent cells with circulating water instead of still water?

Is there any difference of transformation efficiency between using plating beads, bending glass rod and plating loop?

Is 1 second vortex critical for mixture the DNA?

Will heat shock affect the transformation efficiency?

Factors Affecting Transformation Efficiency

Thawing methods

Size of plasmid

Heat shock treatment

Plating methods

Concentration of antibiotic

High Fidelity PCR amplification