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SMOBIO/[TQ1200]ExcelTaq公司™ 2X快速Q-PCR主混合物（SYBR，无ROX），200 Rxn/SYBR，无ROX），200 Rxn
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Description
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program.
With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Features
High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye
Storage
Protect from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
Fast Hot Start
The overlapped amplification curves from different hot start duration display fast activation of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).
The initial activation step was set as 2,5, and 10 minutes. TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) contains a special hot-start Taq polymerase that omits time-consuming initial activation step required by chemically modified hot start Taq polymerases (Brand A).
High Sensitivity
The amplification curves from different amounts of cDNA display high sensitivity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).
The sensitivity assay of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) was examined with serial diluted cDNA from 25 ng to 1.4 fg as templates. The equal intervals of amplification curves also displayed the high precision of TQ1200 in target quantification.
Low Background
The single peak of melting curve displays high specificity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).
The post-amplification melting-curve analysis was examined with 5 ng cDNA as template, abnormally high concentration of primers (400 nM), and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) or qPCR master mix from brand A as reagents.
High Stability
The overlapped amplification curves and melting curves from different storage conditions display high stability of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).
Stability was analyzed via real-time PCR amplification with 1 ng cDNA as template, and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) stored at -20°C, 4°C, 22°C and 37°C for 30 days as reagents.
Suitable for Fast Program
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Smart Blue Contrast Dye
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Contents
Component | Volume | Cat. No |
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | 2 x 1 ml | TQ1200 (200 Rxn) |
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | 5 x 1 ml | TQ1201 (500 Rxn) |
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
Manual
Manual_TQ1200_ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)
SDS
SDS_TQ1200
Flyer
SMOBIO’s Q-PCR Master Mix- Instrument Compatibility
ExcelTaq™ 2X Fast Q-PCR Master Mix
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
Instrument Compatibility* | |||||
Applied Biosystems system: | |||||
5700, 7000, 7300, 7700, and 7900HT | Δ | V | Δ | V | V |
StepOne, StepOnePlus | Δ | V | Δ | V | V |
7500, 7500 Fast, QuantStudio, ViiA7 | Δ | Δ | Δ | Δ | Δ |
BioRad system: | |||||
CFX96 , CFX384 | V | V | V | V | V |
iQ5 | V | V | V | V | V |
Opticon, Opticon 2, Chromo 4 | V | V | V | V | V |
Cepheid system: | |||||
Smart Cycler | V | V | V | V | V |
Eppendorf system: | |||||
Mastercycler ep realplex | V | V | V | V | V |
Roche system: | |||||
LightCycler 480, Nano | V | V | V | V | V |
QIAgen system: | |||||
Rotor-Gene™ 3000, 6000, Q | V | V | V | V | V |
Agilent (Stratagene) system: | |||||
MX3000P, 3005P, 4000P | Δ | Δ | Δ | Δ | Δ |
Illumina system: | |||||
Eco | V | V | V | V | V |
Can you provide the results that using ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX, TQ1200) with different instruments?
Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)
Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore
Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y
PMCID: PMC6757040
Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.
Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore
Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328
Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells
Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056
PMCID: PMC5522218
Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031
PMCID: PMC5706436
Loss of endogenous estrogen increases cardiac toxicity by doxorubicin
Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong
Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146
ExcelTaq™ 2X Q-PCR Master Mix
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
