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SMOBIO/[TQ1210]卓越™ 2X快速Q-PCR主混合物（SYBR，ROX），200 Rxn/SYBR，ROX），200 Rxn"},{"src":"","
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Description
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Features
High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye
With ROX Reference Dye
Storage
Protect from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
Fast Hot Start
The standard curves were examined using fast program with cDNA ranging from 200 fg to 20 ng as template. The efficiency was calculated according to the slope of standard curve. The efficiency of TQ1210 is approximately 100% showing that TQ1210 is more compatible with fast program as compared to brand A (efficiency: 71.9).
High Sensitivity
The sensitivity assay of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) was examined with serially diluted cDNA from 25 ng to 1.4 fg as templates. The amplification curves display that TQ1210 is capable to amplify template as little as 1.4 fg, and over a wide range of template usage. The sensitivity of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) was higher than that of brand P and brand A. The sensitivity assay was examined with 0.2 fg cDNA as template.
Low Background
The post-amplification melt-curve analyzed with relatively higher concentration of primers (400 nM) reveals the specificity of qPCR reagents. The multiple peaks of melt curve display a nonspecific and a target PCR products. The ratio of nonspecific PCR product to target PCR product represents the specificity of qPCR reagents. The ratio analyzed using TQ1210 (0.5 to 1) was lower than the ratio analyzed from brand P (2 to 1) and A (0.8 to 1), showing high specificity of TQ1210.
High Stability
Stability was analyzed via real-time PCR amplification with 2 ng, 20 pg and 0.2 pg cDNA as template, and TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) stored at -20°C or 37°C for 4 weeks as reagents. The overlapped amplification curves of qPCR reactions from different storage conditions display excellent stability of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX). Inconsistent Ct value and decreased fluorescent intensity were observed in qPCR master mix from brand P.
Suitable for Fast Program
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Smart Blue Contrast Dye
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Contents
Component | Volume | Cat. No |
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | 2 x 1 ml | TQ1210 (200 Rxn) |
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | 5 x 1 ml | TQ1211 (500 Rxn) |
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
Manual
Manual_TQ1210_ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)
SDS
SDS_TQ1210
Flyer
SMOBIO’s Q-PCR Master Mix- Instrument Compatibility
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
Instrument Compatibility* | |||||
Applied Biosystems system: | |||||
5700, 7000, 7300, 7700, and 7900HT | Δ | V | Δ | V | V |
StepOne, StepOnePlus | Δ | V | Δ | V | V |
7500, 7500 Fast, QuantStudio, ViiA7 | Δ | Δ | Δ | Δ | Δ |
BioRad system: | |||||
CFX96 , CFX384 | V | V | V | V | V |
iQ5 | V | V | V | V | V |
Opticon, Opticon 2, Chromo 4 | V | V | V | V | V |
Cepheid system: | |||||
Smart Cycler | V | V | V | V | V |
Eppendorf system: | |||||
Mastercycler ep realplex | V | V | V | V | V |
Roche system: | |||||
LightCycler 480, Nano | V | V | V | V | V |
QIAgen system: | |||||
Rotor-Gene™ 3000, 6000, Q | V | V | V | V | V |
Agilent (Stratagene) system: | |||||
MX3000P, 3005P, 4000P | Δ | Δ | Δ | Δ | Δ |
Illumina system: | |||||
Eco | V | V | V | V | V |
Can you provide the results that using ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX, TQ1210) with different instruments?
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Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore
Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328
Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells
Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056
PMCID: PMC5522218
Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031
PMCID: PMC5706436
Loss of endogenous estrogen increases cardiac toxicity by doxorubicin
Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong
Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146
ExcelTaq™ 2X Q-PCR Master Mix
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
