SMOBIO/[TP2100] ExcelTaq™ 5X Blood Direct PCR Master Mix Kit, 200 RXN/s)"}]]> </ul> <a -免疫在线蚂蚁淘旗下平台

The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.

Features

5"→3" DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High throughput PCR
High yield PCR
High reproducibility, with reduced pipetting errors
Compatible with most anticoagulants

Applications

Direct amplification of DNA from blood samples
High throughput screening without DNA purification
Suitable for multiplex PCR

Storage

Caution: Avoid Multiple Freeze/Thaw Cycles

4°C for 6 months-20°C for 24 months

Compatible with most anticoagulants

ExcelTaq™ 5X Blood Direct PCR Master Mix amplified 200 bp from differently treated blood using CCR5 specific primers. (M: DM2100)

Component

Volume

5X Blood Direct PCR Master Mix

2 x 1 ml

Positive Control Primers (10 μM,each)

25 μl

CCR5 control primer sequence

CCR5-F:5"-CTCCCAGGAATCATCTTTACC-3"

CCR5-R:5"-TCATTTCGACACCGAAGCAG-3"

Storage

Caution: Avoid Multiple Freeze/Thaw Cycles

4°C for 6 months-20°C for 24 months

Manual

Manual_TP2100_ExcelTaq™ 5X Blood Direct PCR Master Mix Kit

SDS

SDS_TP2100

Recommended PCR Condition

Blood

3μl

Forward primer

0.1– 0.5 µM

Reverse primer

0.1– 0.5 µM

5X Blood Direct PCR Master Mix

10μl

H₂O

to50 μl

Total volume

50µl

Note: Mix well before PCR Reaction

Recommended PCRProgram

Steps

Temp.

Time

Cycles

Templatedenature

94°C

3 min

Denature

94°C

30 sec

25-40

Annealing

50-68°C**

30 sec

Extension

72°C

60 sec/kb

Final extension

72°C

3 min

*Optimal PCR condition variesaccording to primers’ thermodynamic properties.

Post amplificationanalysis

Centrifuge PCR reaction mixture at 1000 × g for 1 ~ 3 minutes. Load only the clear supernatant of the PCRproducts for gel electrophoresis analysis.

[TP2000] ExcelTaq™ Blood Direct DNA Polymerase

High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity

[TP5000] ExcelTaq™ Hot Start II DNA Polymerase

Aptamer-based hot start PCR
Reversible enzyme inactivation
Omits extra enzyme activation step
Convenient for room temperature PCR set-up
High yield and specificity of target amplicons
Wide range of amplicon length (up to 10 kb)
High sensitivity (as low as 1 fg of plasmid)

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR

品牌介绍

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公司介绍

品牌分类

DNA梯子蛋白质标记物前盖尔斯 n. 照明者; n. 核酸; cDNA合成和RT-PCR 蛋白质污渍 DNA扩增; n. 无性（繁殖）系化，纯系化;

DNA染料

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Compatible with most anticoagulants

Contents

[TP2000] ExcelTaq™ Blood Direct DNA Polymerase

[TP5000] ExcelTaq™ Hot Start II DNA Polymerase

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix